Characterisation of diarrhoeagenic Escherichia coli clones by ribotyping and ERIC-PCR

Detection of diarrhoeagenic Escherichia coli virulence genes by multiplex-PCR method and their antibiotic susceptibility profile

Acute diarrhoeal disease is a public health problem and an important cause of morbidity and mortality, particularly in developing countries. The etiology is varied, and the diarrheagenic Escherichiacolipathotypes are most important. Our objectives were detection of Diarrhoeagenic E. coli virulence genes by Multiplex-PCR method and their antimicrobial susceptibility profile. Fifty five E. Coli s...

Enterobacterial repetitive intergenic consensus (ERIC) sequences in Escherichia coli: Evolution and implications for ERIC-PCR.

Enterobacterial repetitive intergenic consensus (ERIC) sequences are 127-bp imperfect palindromes that occur in multiple copies in the genomes of enteric bacteria and vibrios. Here we investigate the distribution of these elements in the complete genome sequences of nine Escherichia coli (including Shigella species) strains. There is a significant tendency for copies to be adjacent to more high...

Molecular typing of avian Escherichia coli isolates by enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR)

BACKGROUND: Colibacillosis is one of the most economically important diseases of poultry worldwide. OBJECTIVES: This study was conducted to examine the clonal relatedness and typing of 95 avian Escherichia coli isolates by ERIC-PCR. METHODS: Sixty-three E. coli isolates from two common manifestations of colibacillosis (yolk sac infection and colisepticemia) and 32 isolates from feces of apparen...

Isolation of enterotoxigenic and enteroaggregative straines of Escherichia coli from chicken carcasses by PCR

The aim of this study was to determine the frequency of enterotoxigenic and enteroaggregative strains ofEscherichia coli in chicken carcasses by polymerase chain reaction (PCR). In this study 63 strains of E. coliwere isolated from 110 samples of chicken carcasses during processing after chilling in the poultry slaughterhouse of Shahrekord. Polymerase chain reaction assays were used to detect t...

Curli Production and ERIC-PCR Fingerprinting of Escherichia coli from Clinical Cases of Bovine Mastitis